Compatibility between Clonostachys isolates with a view to mixed inocula for biocontrol.
Identifieur interne : 001796 ( Main/Exploration ); précédent : 001795; suivant : 001797Compatibility between Clonostachys isolates with a view to mixed inocula for biocontrol.
Auteurs : G Martijn Ten Hoopen [Costa Rica] ; André George ; Adolfo Martinez ; Tim Stirrup ; Julie Flood ; Ulrike KraussSource :
- Mycologia [ 0027-5514 ]
Descripteurs français
- KwdFr :
- MESH :
- croissance et développement : Phytophthora.
- isolement et purification : Gliocladium, Phytophthora.
- méthodes : Lutte biologique contre les nuisibles.
- pathogénicité : Gliocladium, Phytophthora.
- physiologie : Gliocladium, Hyphae.
English descriptors
- KwdEn :
- MESH :
- growth & development : Phytophthora.
- isolation & purification : Gliocladium, Phytophthora.
- methods : Pest Control, Biological.
- pathogenicity : Gliocladium, Phytophthora.
- physiology : Gliocladium, Hyphae.
Abstract
To aid the development of compatible biocontrol inocula, a prescreening method for the prediction of compatibility of fungal antagonists was developed. Compatibility between 18 Clonostachys isolates with known antagonistic capabilities against Phytophthora palmivora was tested using intra- or interisolate pairings (dual cultures) on water agar plates, a hyphal interaction experiment and a modified double host-range experiment. Almost all inter- or intraisolate pairings of Clonostachys isolates showed growth inhibition zones and did not show free hyphal intermingling. A hyphal interaction experiment on water agar demonstrated that the aggressiveness of a Clonostachys isolate and its susceptibility to mycoparasitism were unrelated phenomena. However the level of aggressiveness and/or susceptibility of an isolate were largely dependant on the isolate with which it was challenged. The degree of growth-inhibition caused by an isolate was unrelated to the hyphal damaged it caused or received. In the double host-range experiment all possible pairs from four Clonostachys isolates were inoculated in different ratios (10 000-fold range) on plates precolonized with one of two P. palmivora isolates. The results showed that antagonistic capabilities of certain combinations were affected by the Clonostachys isolates. The primary host, P. palmivora, did not affect antagonistic capabilities; whereas inoculum ratio did. Of note, it was not possible to predict the outcome of the double host range on the basis of the results of the hyphal interaction experiment. In conclusion the competitive abilities of Clonostachys isolates depend on the partner with which they are applied and less on resource availability. The double host-range test as developed here might provide the most representative tool to date to test compatibility of fungal antagonists to be used in biocontrol inocula. However the link between the results of the double host-range test and field efficacy of biocontrol inocula remains to be investigated.
DOI: 10.3852/08-095
PubMed: 20943520
Affiliations:
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first="Adolfo" last="Martinez">Adolfo Martinez</name></author><author><name sortKey="Stirrup, Tim" sort="Stirrup, Tim" uniqKey="Stirrup T" first="Tim" last="Stirrup">Tim Stirrup</name></author><author><name sortKey="Flood, Julie" sort="Flood, Julie" uniqKey="Flood J" first="Julie" last="Flood">Julie Flood</name></author><author><name sortKey="Krauss, Ulrike" sort="Krauss, Ulrike" uniqKey="Krauss U" first="Ulrike" last="Krauss">Ulrike Krauss</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010 Sep-Oct</date><idno type="RBID">pubmed:20943520</idno><idno type="pmid">20943520</idno><idno type="doi">10.3852/08-095</idno><idno type="wicri:Area/Main/Corpus">001814</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001814</idno><idno type="wicri:Area/Main/Curation">001814</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001814</idno><idno 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sort="Martinez, Adolfo" uniqKey="Martinez A" first="Adolfo" last="Martinez">Adolfo Martinez</name></author><author><name sortKey="Stirrup, Tim" sort="Stirrup, Tim" uniqKey="Stirrup T" first="Tim" last="Stirrup">Tim Stirrup</name></author><author><name sortKey="Flood, Julie" sort="Flood, Julie" uniqKey="Flood J" first="Julie" last="Flood">Julie Flood</name></author><author><name sortKey="Krauss, Ulrike" sort="Krauss, Ulrike" uniqKey="Krauss U" first="Ulrike" last="Krauss">Ulrike Krauss</name></author></analytic><series><title level="j">Mycologia</title><idno type="ISSN">0027-5514</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Gliocladium (isolation & purification)</term><term>Gliocladium (pathogenicity)</term><term>Gliocladium (physiology)</term><term>Hyphae (physiology)</term><term>Pest Control, Biological (methods)</term><term>Phytophthora (growth & development)</term><term>Phytophthora (isolation & purification)</term><term>Phytophthora (pathogenicity)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Gliocladium (isolement et purification)</term><term>Gliocladium (pathogénicité)</term><term>Gliocladium (physiologie)</term><term>Hyphae (physiologie)</term><term>Lutte biologique contre les nuisibles (méthodes)</term><term>Phytophthora (croissance et développement)</term><term>Phytophthora (isolement et purification)</term><term>Phytophthora (pathogénicité)</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Gliocladium</term><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Gliocladium</term><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Pest Control, Biological</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Lutte biologique contre les nuisibles</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Gliocladium</term><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Gliocladium</term><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Gliocladium</term><term>Hyphae</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Gliocladium</term><term>Hyphae</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To aid the development of compatible biocontrol inocula, a prescreening method for the prediction of compatibility of fungal antagonists was developed. Compatibility between 18 Clonostachys isolates with known antagonistic capabilities against Phytophthora palmivora was tested using intra- or interisolate pairings (dual cultures) on water agar plates, a hyphal interaction experiment and a modified double host-range experiment. Almost all inter- or intraisolate pairings of Clonostachys isolates showed growth inhibition zones and did not show free hyphal intermingling. A hyphal interaction experiment on water agar demonstrated that the aggressiveness of a Clonostachys isolate and its susceptibility to mycoparasitism were unrelated phenomena. However the level of aggressiveness and/or susceptibility of an isolate were largely dependant on the isolate with which it was challenged. The degree of growth-inhibition caused by an isolate was unrelated to the hyphal damaged it caused or received. In the double host-range experiment all possible pairs from four Clonostachys isolates were inoculated in different ratios (10 000-fold range) on plates precolonized with one of two P. palmivora isolates. The results showed that antagonistic capabilities of certain combinations were affected by the Clonostachys isolates. The primary host, P. palmivora, did not affect antagonistic capabilities; whereas inoculum ratio did. Of note, it was not possible to predict the outcome of the double host range on the basis of the results of the hyphal interaction experiment. In conclusion the competitive abilities of Clonostachys isolates depend on the partner with which they are applied and less on resource availability. The double host-range test as developed here might provide the most representative tool to date to test compatibility of fungal antagonists to be used in biocontrol inocula. However the link between the results of the double host-range test and field efficacy of biocontrol inocula remains to be investigated.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20943520</PMID><DateCompleted><Year>2010</Year><Month>11</Month><Day>16</Day></DateCompleted><DateRevised><Year>2010</Year><Month>10</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0027-5514</ISSN><JournalIssue CitedMedium="Print"><Volume>102</Volume><Issue>5</Issue><PubDate><MedlineDate>2010 Sep-Oct</MedlineDate></PubDate></JournalIssue><Title>Mycologia</Title><ISOAbbreviation>Mycologia</ISOAbbreviation></Journal><ArticleTitle>Compatibility between Clonostachys isolates with a view to mixed inocula for biocontrol.</ArticleTitle><Pagination><MedlinePgn>1204-15</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3852/08-095</ELocationID><Abstract><AbstractText>To aid the development of compatible biocontrol inocula, a prescreening method for the prediction of compatibility of fungal antagonists was developed. Compatibility between 18 Clonostachys isolates with known antagonistic capabilities against Phytophthora palmivora was tested using intra- or interisolate pairings (dual cultures) on water agar plates, a hyphal interaction experiment and a modified double host-range experiment. Almost all inter- or intraisolate pairings of Clonostachys isolates showed growth inhibition zones and did not show free hyphal intermingling. A hyphal interaction experiment on water agar demonstrated that the aggressiveness of a Clonostachys isolate and its susceptibility to mycoparasitism were unrelated phenomena. However the level of aggressiveness and/or susceptibility of an isolate were largely dependant on the isolate with which it was challenged. The degree of growth-inhibition caused by an isolate was unrelated to the hyphal damaged it caused or received. In the double host-range experiment all possible pairs from four Clonostachys isolates were inoculated in different ratios (10 000-fold range) on plates precolonized with one of two P. palmivora isolates. The results showed that antagonistic capabilities of certain combinations were affected by the Clonostachys isolates. The primary host, P. palmivora, did not affect antagonistic capabilities; whereas inoculum ratio did. Of note, it was not possible to predict the outcome of the double host range on the basis of the results of the hyphal interaction experiment. In conclusion the competitive abilities of Clonostachys isolates depend on the partner with which they are applied and less on resource availability. The double host-range test as developed here might provide the most representative tool to date to test compatibility of fungal antagonists to be used in biocontrol inocula. However the link between the results of the double host-range test and field efficacy of biocontrol inocula remains to be investigated.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>ten Hoopen</LastName><ForeName>G Martijn</ForeName><Initials>GM</Initials><AffiliationInfo><Affiliation>CABI-CATIE-DGIS, c/o Centro Agronómico Tropical de Investigación y Enseñanza (CATIE), 7170 Turrialba, Costa Rica. tenhoopen@cirad.fr</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>George</LastName><ForeName>André</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Martinez</LastName><ForeName>Adolfo</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Stirrup</LastName><ForeName>Tim</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Flood</LastName><ForeName>Julie</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Krauss</LastName><ForeName>Ulrike</ForeName><Initials>U</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Mycologia</MedlineTA><NlmUniqueID>0400764</NlmUniqueID><ISSNLinking>0027-5514</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D020462" MajorTopicYN="N">Gliocladium</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D025301" MajorTopicYN="N">Hyphae</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010572" MajorTopicYN="N">Pest Control, Biological</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>10</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>10</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>11</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20943520</ArticleId><ArticleId IdType="pii">08-095</ArticleId><ArticleId IdType="doi">10.3852/08-095</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Costa Rica</li></country></list><tree><noCountry><name sortKey="Flood, Julie" sort="Flood, Julie" uniqKey="Flood J" first="Julie" last="Flood">Julie Flood</name><name sortKey="George, Andre" sort="George, Andre" uniqKey="George A" first="André" last="George">André George</name><name sortKey="Krauss, Ulrike" sort="Krauss, Ulrike" uniqKey="Krauss U" first="Ulrike" last="Krauss">Ulrike Krauss</name><name sortKey="Martinez, Adolfo" sort="Martinez, Adolfo" uniqKey="Martinez A" first="Adolfo" last="Martinez">Adolfo Martinez</name><name sortKey="Stirrup, Tim" sort="Stirrup, Tim" uniqKey="Stirrup T" first="Tim" last="Stirrup">Tim Stirrup</name></noCountry><country name="Costa Rica"><noRegion><name sortKey="Ten Hoopen, G Martijn" sort="Ten Hoopen, G Martijn" uniqKey="Ten Hoopen G" first="G Martijn" last="Ten Hoopen">G Martijn Ten Hoopen</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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